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Nicotine metabolite urine test to confirm tobacco use or smoke exposure status

Nicotine & Tobacco Metabolite Urine Test

Nicotine and Expanded Metabolites, LC/MS/MS, Urine

  • Detect recent tobacco or nicotine exposure
  • Confirm smoke-free progress after quitting
  • Screen for secondhand smoke exposure levels
$394
First results as soon as overnight*

All results normal? Get your money back. First-time customers only. File a support ticket titled “Money Back Guarantee” with your order number within 15 days of receiving your results. STD, Sickle Cell, and gift orders aren’t eligible. Full terms apply. guarantee
No questions asked refunds
LegitScript certified

Prescription & all lab fees included

Go directly to the lab, no extra fees

Doctor's guidance included

Easy to understand results

Fast & convenient

Lab visit as quick as 10 mins in & out

Prescription & all lab fees included

Go directly to the lab, no extra fees

Doctor's guidance included

Easy to understand results

Fast & convenient

Lab visit as quick as 10 mins in & out

All results normal? Get your money back. First-time customers only. File a support ticket titled “Money Back Guarantee” with your order number within 15 days of receiving your results. STD, Sickle Cell, and gift orders aren’t eligible. Full terms apply. guarantee
No questions asked refunds
LegitScript certified

What's this test for?

This test is ideal if you're trying to confirm tobacco use or exposure, whether for personal health tracking, a workplace requirement, or an insurance screening. It helps if you need to know whether nicotine or tobacco byproducts are present in your system, and whether that came from active smoking, smokeless tobacco, secondhand smoke, or a nicotine replacement product like a patch or gum. It's also useful if you're trying to verify that you've successfully quit and stayed tobacco-free.

What happens after I get my results?

Your results show up in your online account with each value explained alongside its normal range, so you can see clearly whether nicotine, its metabolites, or anabasine were detected. If tobacco exposure is confirmed and you're trying to quit, next steps might include cutting back gradually, avoiding secondhand smoke environments, and retesting after a few weeks to check your progress. You can download a standard lab report straight from your account, and if you test periodically, the trends feature lets you track your tobacco-free progress over time.

Who should consider this test?

This test is a good fit for people trying to quit smoking who want objective proof of their progress, individuals subject to workplace or insurance tobacco-use screening, and anyone concerned about secondhand smoke exposure. It's also useful for people using nicotine replacement products who want to distinguish that use from actual tobacco exposure, since anabasine only shows up with real tobacco.

What symptoms indicate I may need this test?
You might consider this test if you're experiencing symptoms tied to tobacco use, like shortness of breath, persistent cough, reduced stamina, or trouble with smell and taste. It's also worth testing if you've been regularly around smokers and are noticing similar symptoms from secondhand exposure. Even without symptoms, this test is commonly used simply to verify tobacco-free status.
When will I get my results?
Results are typically ready within about 7 days of your sample being processed at the lab. You'll get a notification once your results are posted, so you can log in and review them without needing to check repeatedly.
How can I naturally support quitting tobacco use?
Simple steps that help include staying hydrated to help your body clear nicotine metabolites faster, getting regular exercise to manage cravings and stress, and avoiding triggers like alcohol or environments where others are smoking. Nicotine replacement products, chewing gum, and keeping your hands busy with a distraction can also reduce the urge to smoke. Retesting after a few weeks can help you see measurable progress, which often reinforces motivation to stay tobacco-free.
What does a positive result for anabasine mean?
A positive anabasine result means you've been exposed to actual tobacco, either through smoking, smokeless tobacco, or secondhand smoke, rather than just a nicotine replacement product. Since anabasine is only found in the tobacco plant itself, it helps distinguish real tobacco use from patches or gum. If you're using a replacement product and get a positive anabasine result, it likely means there's also been tobacco exposure from another source.
What causes a positive nicotine or metabolite result?
A positive result usually comes from directly smoking or using smokeless tobacco, but it can also happen from regular secondhand smoke exposure in enclosed spaces like a car or home. Nicotine replacement products like patches, gum, or lozenges will show nicotine and its metabolites too, but not anabasine. The level detected can give a rough sense of how recent or how heavy the exposure was, since nicotine itself clears the body quickly while metabolites linger longer.
How often should I retest?
If you're actively trying to quit, retesting every few weeks can help you track your progress and stay motivated. For workplace or insurance purposes, follow the specific schedule required by that program. If you're just confirming a one-time exposure question, a single test is usually enough.
What should I do if my results show tobacco exposure I didn't expect?
If you get a positive result you didn't expect, think about recent environments you've been in, since secondhand smoke can trigger detectable levels even without direct use. Consider avoiding those spaces going forward and retest in a few weeks to see if the levels change. If you're unsure how a nicotine replacement product might be affecting your results, remember that a positive anabasine result specifically points to actual tobacco exposure rather than the replacement product.
How’s this work?
Getting your blood test with us is easy, private & backed by the power of science.

Long story short:
  1. In the test options, use the filters or search box to narrow your choices and find the test you want.
  2. If you need a hand navigating through options, text our super friendly support team at 754-799-7833, and we'll provide tailored suggestions to help you find the ideal test.
  3. Once your order is placed, we’ll create your doctor’s lab order remotely, without any need for you to make a trip to a doctor in person or to talk to the doctor. Expect to receive your doctor’s lab order right in your inbox. It will also be in your patient portal if you need to find it later. We’ll also include instructions regarding fasting and other requirements for your test. Your patient portal will be auto-created hassle-free during checkout.
  4. If this is your first time ordering, you'll be prompted to create a password for instant login access to your patient portal. It's a quick way to conveniently access your orders and results whenever you want.
  5. When you’re ready, visit one of our 4,000 locations. Just bring your ID – no printing or faxing of your lab order is needed, as your order and details will already be in the lab system.
  6. Note that most lab locations don’t accept walk-ins, so it’s best to book an appointment in advance. Don’t worry – we’ll provide detailed instructions along the way.
  7. You’ll get your results via email & SMS and dive into understanding your body better. Yup, that easy!
  8. If you have any questions, please text us at 754-799-7833 or email [email protected] and we'll gladly help you.
How do I know which test to get?
In the test options, find the test you want. If you’re not sure which test to get, we can lend a hand in finding the right option.

Just text or call us at 754-799-7833, email us at [email protected], and we'll gladly help you. We've got your back and reply quickly.
Is it possible to make changes to my lab order if I made a mistake with the name, date of birth, or any other details?
Absolutely! We totally understand that errors can happen. No worries, we're here to help you.

Just reach out to us via text at 754-799-7833 or shoot us an email at [email protected]. Remember to include your order number and let us know the correct information you’d like to update. Our awesome team will jump right in and make sure everything is sorted out and accurate for you.

There are no changes necessary if your address is wrong on the requisition though. We don’t mail anything out.

Our lab requires an address to be listed to generate an order.
Do you accept health insurance?
Only HSA & FSA is accepted.

Our services are strictly self-pay and cannot be submitted to your health insurance provider except for Health Savings Accounts or Flexible Savings Accounts.

This policy applies to all insurance companies, including federal health insurance programs like Medicare.

If you have any questions, please text us at 754-799-7833 or email [email protected].
Can I cancel my order?
Yes.

We get it – sometimes your needs change.

As long as your samples haven’t been collected yet, we’re happy to help you cancel your order.

If you have any further questions, please text us at 754-799-7833 or email [email protected].

You can read more about our cancellation policy here.
How can I find a lab location near me?
During the ordering process, you’ll be able to select a specific lab near you, with no strings attached! You can switch it up later easily and visit any of our authorized locations as long as it’s the same lab company you selected your test for (Quest Diagnostics or Labcorp).

Before you proceed with your order, feel free to browse through all our lab locations here. This will give you the peace of mind of knowing that there's a lab nearby your home, office or your favorite gym.

If you have any questions, please text us at 754-799-7833 or email [email protected] and we'll gladly help you.

Here’s why 1,000,000+ customers chose us for affordable hassle-free private blood testing.

You get clinical lab reports & simplified health insights.
Save time & gas, go straight to the lab. Our doctors cover the rest.
Results start landing the next day after your lab visit.*
We don’t sell or share your data. Your time & privacy matter.
US-licensed doctors have your back if things get unclear.
No hidden fees, nice discounts for regular checkups.
Save a trip to the doctor, go directly to the lab
Get lab order in minutes, results come overnight*
Results explained in simple language
Reviewed by US licensed doctors
Fast & confidential, we never sell or share your data
No insurance needed, transparent pricing
Demo results
Nicotine Metabolites (Cotinine) and Anabasine Negative
Demo results
Negative (No Tobacco Exposure) Positive (Tobacco Exposure Detected)
Negative

What this means

A negative result means no nicotine, metabolites, or anabasine were detected in your urine, indicating no recent tobacco use or exposure. This is the expected result for non-smokers and successfully quit individuals who haven't been around secondhand smoke recently.

* Regular blood test results (e.g., CBC) typically start arriving the next business day after sample collection. More advanced tests may take longer due to complexity.
Here’s how easy it is to get blood work done on your terms
Choose a checkup
Order your test Save a trip to the doctor. Your results will come with clear, friendly explanations. Takes 2 minutes
Book a lab
Book an appointment Visit any of our 4,000 nearby lab locations to have your samples collected. Book or walk-in
Choose a checkup
Get results Receive a clear, easy-to-digest health report by email & SMS within a few business days. Get answers, fast

Find a lab near you

Search Quest Diagnostics and LabCorp locations available through Private MD Labs.

Find a lab

Nicotine & Tobacco Metabolite Urine Test

  • Detect recent tobacco or nicotine exposure
  • Confirm smoke-free progress after quitting
  • Screen for secondhand smoke exposure levels
$394
Still got questions? Text our super-cool support team at 754-799-7833 to get help finding your ideal checkup.
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For informational purposes only. Not medical advice.