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Nicotine and cotinine urine test for smoking cessation verification and tobacco exposure monitoring

Nicotine Test for Smoking Cessation and Tobacco Exposure

Nicotine and Cotinine, LC/MS/MS, Urine

Google
3,138 reviews
  • Detect nicotine levels to manage cravings
  • Verify smoking cessation progress accurately
  • Highlight exposure to secondhand smoke clearly
First results as soon as overnight*

90 day happiness guarantee

We stand behind our service

Prescription & fees included

Go directly to the lab, no extra fees

Easy to understand results

With friendly doctor's notes & guidance

Fast & convenient

Lab visit as quick as 10 mins in & out

90 day happiness guarantee

We stand behind our service

Prescription & fees included

Go directly to the lab, no extra fees

Easy to understand results

With friendly doctor's notes & guidance

Fast & convenient

Lab visit as quick as 10 mins in & out

What's this test for?

This test is ideal if you're trying to quit smoking and want to verify your progress, need documentation of tobacco-free status for insurance or employment, or are concerned about exposure to secondhand smoke. It measures nicotine and its metabolite cotinine in your urine to confirm whether you've been exposed to tobacco recently or over the past several days. Many people use this test to track their smoking cessation journey or to prove they're tobacco-free.

What's included in this test?

Cotinine, Urine Nicotine, Urine

This test measures both nicotine and cotinine levels in your urine. Nicotine has a very short half-life of about 40 minutes and indicates recent tobacco exposure within hours, while cotinine is the major metabolite that lasts much longer (24-hour half-life) and can be detected for several days after tobacco use. Together, these measurements provide a complete picture of both recent and ongoing tobacco exposure, making it useful for verifying smoking cessation or identifying secondhand smoke exposure.

What happens after I get my results?

You'll receive detailed doctor notes in simple language explaining what your nicotine and cotinine levels mean for your tobacco exposure status. These notes include personalized recommendations like strategies to avoid secondhand smoke, tips for maintaining tobacco-free status, or whether you should consider additional support for smoking cessation. You'll also get the standard lab report and can track your levels over time using our trends feature to monitor your progress as you quit smoking or stay tobacco-free.

When will I get my results?
Results are typically available within 3-5 business days after your sample is received by the lab. You'll be notified by email as soon as your results are ready, and you can access them securely through your online account along with the detailed doctor notes and standard lab report.
Who should consider getting this test?
This test is valuable for anyone actively quitting smoking who wants objective proof of their tobacco-free status, people who need documentation for life insurance applications or employment screenings, and individuals concerned about exposure to secondhand smoke at home or work. It's also useful for parents who want to verify their teenagers aren't using tobacco products, or anyone participating in smoking cessation programs who wants to track their progress.
What symptoms indicate I may need this test?
You might consider this test if you've recently quit smoking and want confirmation that nicotine has cleared your system, if you're experiencing health issues that could be related to tobacco exposure, or if you live or work in environments with heavy secondhand smoke. It's also relevant if you're applying for lower insurance premiums as a non-smoker, or if you need to document tobacco-free status for medical procedures or employment requirements.
How can I naturally support tobacco cessation and eliminate nicotine from my body?
Staying well-hydrated by drinking plenty of water helps flush nicotine and cotinine from your system more quickly. Regular cardiovascular exercise increases metabolism and circulation, speeding up the elimination process. Eating antioxidant-rich foods like fruits and vegetables supports your body's detoxification systems, while adequate sleep and stress management techniques like deep breathing or meditation can reduce cravings and support your quit journey.
What is cotinine and why does it matter?
Cotinine is the primary metabolite that your body produces when it breaks down nicotine. It matters because it stays in your system much longer than nicotine itself (up to several days versus just hours), making it a more reliable marker for determining tobacco exposure over time. Cotinine testing is considered the gold standard for verifying smoking status because it's less affected by the timing of your last cigarette and provides a more accurate picture of your overall tobacco use or exposure.
What does a positive result mean?
A positive result means that nicotine or cotinine was detected in your urine, indicating recent tobacco exposure. This could mean you've smoked or used tobacco products within the detection window, been exposed to significant secondhand smoke, or used nicotine replacement products like patches or gum. The levels detected can help distinguish between active smoking, secondhand exposure, or nicotine replacement therapy use.
How often should I retest my nicotine and cotinine levels?
If you're actively quitting smoking, testing 3-7 days after your last cigarette can confirm that cotinine has cleared your system. For ongoing verification of tobacco-free status during a quit program, monthly testing for the first few months can provide motivation and accountability. If you need to maintain documented proof of non-smoking status for insurance or employment, annual or semi-annual testing is typically sufficient once you've established a tobacco-free pattern.
How’s this work?
Getting your blood test with us is easy, private & backed by the power of science.

Long story short:
  1. In the test options, use the filters or search box to narrow your choices and find the test you want.
  2. If you need a hand navigating through options, text our super friendly support team at 754-799-7833, and we'll provide tailored suggestions to help you find the ideal test.
  3. Once your order is placed, we’ll create your doctor’s lab order remotely, without any need for you to make a trip to a doctor in person or to talk to the doctor. Expect to receive your doctor’s lab order right in your inbox. It will also be in your patient portal if you need to find it later. We’ll also include instructions regarding fasting and other requirements for your test. Your patient portal will be auto-created hassle-free during checkout.
  4. If this is your first time ordering, you'll be prompted to create a password for instant login access to your patient portal. It's a quick way to conveniently access your orders and results whenever you want.
  5. When you’re ready, visit one of our 4,000 locations. Just bring your ID – no printing or faxing of your lab order is needed, as your order and details will already be in the lab system.
  6. Note that most lab locations don’t accept walk-ins, so it’s best to book an appointment in advance. Don’t worry – we’ll provide detailed instructions along the way.
  7. You’ll get your results via email & SMS and dive into understanding your body better. Yup, that easy!
  8. If you have any questions, please text us at 754-799-7833 or email [email protected] and we'll gladly help you.
How do I know which test to get?
In the test options, find the test you want. If you’re not sure which test to get, we can lend a hand in finding the right option.

Just text or call us at 754-799-7833, email us at [email protected], and we'll gladly help you. We've got your back and reply quickly.
Is it possible to make changes to my lab order if I made a mistake with the name, date of birth, or any other details?
Absolutely! We totally understand that errors can happen. No worries, we're here to help you.

Just reach out to us via text at 754-799-7833 or shoot us an email at [email protected]. Remember to include your order number and let us know the correct information you’d like to update. Our awesome team will jump right in and make sure everything is sorted out and accurate for you.

There are no changes necessary if your address is wrong on the requisition though. We don’t mail anything out.

Our lab requires an address to be listed to generate an order.
Do you accept health insurance?
Only HSA & FSA is accepted.

Our services are strictly self-pay and cannot be submitted to your health insurance provider except for Health Savings Accounts or Flexible Savings Accounts.

This policy applies to all insurance companies, including federal health insurance programs like Medicare.

If you have any questions, please text us at 754-799-7833 or email [email protected].
Can I cancel my order?
Yes.

We get it – sometimes your needs change.

As long as your samples haven’t been collected yet, we’re happy to help you cancel your order.

If you have any further questions, please text us at 754-799-7833 or email [email protected].

You can read more about our cancellation policy here.
How can I find a lab location near me?
During the ordering process, you’ll be able to select a specific lab near you, with no strings attached! You can switch it up later easily and visit any of our authorized locations as long as it’s the same lab company you selected your test for (Quest Diagnostics or Labcorp).

Before you proceed with your order, feel free to browse through all our lab locations here. This will give you the peace of mind of knowing that there's a lab nearby your home, office or your favorite gym.

If you have any questions, please text us at 754-799-7833 or email [email protected] and we'll gladly help you.

Here’s why 1,000,000+ customers chose us for affordable hassle-free private blood testing.

You get clinical lab reports & simplified health insights.
Save time & gas, go straight to the lab. Our doctors cover the rest.
Results start landing the next day after your lab visit.*
We don’t sell or share your data. Your time & privacy matter.
US-licensed doctors have your back if things get unclear.
No hidden fees, nice discounts for regular checkups.
Save a trip to the doctor, go directly to the lab
Instant orders, results often overnight*
Results explained in simple language
Reviewed by US licensed doctors
Fast & confidential, we never sell or share your data
No insurance needed, transparent pricing
Demo results
Cotinine, Urine Negative
Demo results
Negative Positive
Negative

What this means

Your cotinine test came back negative, meaning no cotinine was detected in your urine. This confirms that you have not been exposed to tobacco smoke recently and indicates successful tobacco-free status, whether from quitting smoking or avoiding secondhand smoke exposure.

* Regular blood test results (e.g., CBC) typically start arriving the next business day after sample collection. More complex tests, such as hormone panels, may take up to 10–15 business days due to their complexity.
Here’s how easy it is to get blood work done on your terms
Choose a checkup
Order your test Save a trip to the doctor. Your results will come with clear, friendly explanations. Takes 2 minutes
Book a lab
Book an appointment Visit any of our 4,000 nearby lab locations to have your samples collected. Book or walk-in
Choose a checkup
Get results Receive a clear, easy-to-digest health report by email & SMS within a few business days. Get answers, fast

Nicotine Test for Smoking Cessation and Tobacco Exposure

Google
3,138 reviews
  • Detect nicotine levels to manage cravings
  • Verify smoking cessation progress accurately
  • Highlight exposure to secondhand smoke clearly

1,000,000+ blood tests later, here's what our customers say

Google reviews 3138 reviews Next
Still got questions? Text our super-cool support team at 754-799-7833 to get help finding your ideal checkup.