Understanding Lab Tests for Vitamin D Deficiency

Written by Private MD Labs Medical Writing Team • Last Updated: September 8, 2025

Vitamin D deficiency occurs when your body doesn't have adequate levels of vitamin D, an essential nutrient crucial for bone health, immune function, and overall well-being. Blood testing is the primary method to accurately diagnose vitamin D deficiency and assess your vitamin D status.

Top Recommended Test

Vitamin D, 1,25-Dihydroxy, LC/MS/MS

This comprehensive test measures the active form of vitamin D in your blood, including both vitamin D2 and D3 metabolites. Low levels can indicate deficiency that may lead to bone diseases like rickets in children and osteomalacia in adults, as well as bone pain and muscle weakness.

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$95

Why Vitamin D Testing is Essential

Vitamin D deficiency can cause bone pain, muscle weakness, and increase the risk of bone diseases like rickets in children and osteomalacia in adults. Since symptoms can be subtle or overlap with other conditions, blood tests provide the definitive measurement needed to confirm deficiency and guide appropriate supplementation.

Other Relevant Tests

Post COVID-19 Expanded Panel

This panel includes vitamin D testing among other important markers. Low vitamin D levels detected through this comprehensive panel can indicate deficiency, which may manifest as bone pain and muscle weakness, conditions that can be particularly relevant for post-COVID recovery.

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$174

Understanding Your Results

Vitamin D blood tests measure the concentration of vitamin D metabolites in your bloodstream. Low levels indicate deficiency, which your healthcare provider can address through dietary changes, increased sun exposure, or vitamin D supplementation. Regular monitoring helps ensure optimal vitamin D status for bone health and overall wellness.

Important Considerations

The information here is for educational purposes only and is not a substitute for professional medical advice. Always consult with a healthcare provider for diagnosis and treatment.

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