Systemic Lupus Erythematosus (SLE) is a chronic autoimmune disease where the immune system attacks healthy tissues and organs throughout the body. It is caused by the production of autoantibodies, particularly antinuclear antibodies (ANA), along with immune complex formation and complement consumption that triggers widespread inflammation. The ANA Screen IFA with Reflex to Titer and Pattern is the most important initial test for diagnosing SLE because it detects the characteristic autoantibodies present in over 95% of lupus patients.
90-day money-back guarantee
Lab order in minutes
Save a trip to the doctor
Low prices since 2005
Labs within 2 miles
Systemic Lupus Erythematosus is caused by an overactive immune system that produces autoantibodies, particularly antinuclear antibodies (ANA), which attack the body's own healthy tissues. The exact trigger is unknown, but a combination of genetic factors (especially HLA-DRB1 and HLA-DQB1 gene variants), environmental triggers like UV light exposure, infections, and hormonal factors contribute to the disease development. When these autoantibodies form immune complexes, they deposit in various organs and activate the complement system, causing widespread inflammation that can affect the skin, joints, kidneys, heart, lungs, and brain.
The ANA Screen IFA with Reflex to Titer and Pattern is the most important initial test for Systemic Lupus Erythematosus because it detects antinuclear antibodies present in over 95% of lupus patients. A positive ANA with specific patterns strongly suggests lupus and triggers further testing. The Complement Component C3c and C4c test is essential for confirming active disease, as low complement levels indicate the immune system is consuming these proteins during inflammation. Additional tests like Interleukin-6 (IL-6) and Beta-2 Microglobulin help assess disease activity and organ involvement, particularly kidney damage. Because lupus is complex, doctors typically use a combination of these blood tests along with your symptoms to make an accurate diagnosis.
You should get tested if you experience a combination of unexplained symptoms including a butterfly-shaped rash across your cheeks and nose, extreme fatigue that does not improve with rest, joint pain and swelling in multiple joints, or unexplained fevers. Testing is especially important if you have persistent symptoms that come and go, sensitivity to sunlight that causes rashes, unexplained hair loss, or if you notice swelling in your legs or around your eyes which could indicate kidney involvement. Women of childbearing age, particularly those with a family history of autoimmune diseases, should consider testing if they experience these symptoms, as lupus is more common in women and people of African, Hispanic, or Asian descent.
Save a trip to the doctor, go directly to the lab
Instant orders, results often overnight*
Results explained in simple language
Reviewed by US licensed doctors
Fast & confidential, we never sell or share your data
No insurance needed, transparent pricing
What this means
Your ANA test came back negative, meaning no antinuclear antibodies were detected in your blood. This is the expected and healthy result, suggesting that an autoimmune condition is less likely to be causing your symptoms.
Recommended actions
Continue monitoring your symptoms and keep a symptom diary to share with your doctor
Focus on an anti-inflammatory diet with plenty of colorful fruits, vegetables, and omega-3 rich foods
Manage stress through regular exercise, adequate sleep, and relaxation techniques
If symptoms persist or worsen, discuss additional testing with your healthcare provider
Not overhyped or overpriced. Just comprehensive blood testing made simple and for everyone.
3303 reviews
Sample results
Your 24/7 Personal Lab Guide
Quick questions:
